缺血预处理对缺血再灌注后兔脊髓磷酸腺苷代谢的影响

目的：研究缺血参处理对缺血再灌注后兔脊髓磷酸腺苷代谢的影响。方法：往置入腹主动脉的Swan-Ganz导管气囊内注气造成兔腰髓缺血模型。将实验兔分为假手术组、缺血组和预处理组。应用反相高效液相色谱方法（reverse phase HPLC),对缺血再灌注后不同时间点腰髓组织中磷酸腺苷（ATP、ADP、AMP）的含量进行检测。结果：和假手术组相比,缺血组兔再灌后各时间点腰髓组织ATP含量有明显下降（P＜0．01）。与缺血组相应时间点相比,预处理组兔再灌注后腰髓组织ATP含量明显提高（P＜0．01）。结论：缺血预处理显著提高缺血再灌注后兔脊髓组织ATP含量,这可能是缺血预处理对脊髓缺血再灌注损伤产生保护作用的机制之一。

The effect of ischemic preconditioning on adenosine phosphate content after spinal cord ischemia in rabbits

Aim: To study the effect of ischemic preconditioning (IP) on adenosine phosphate content after spinal cord ischemia in rabbits. Methods: We established a 1umbar spinal cord ischemia model by inflating the balloon of a 4F Swan Ganz catheter positioned in the abdominal aorta of the rabbits. All rabbits were divided into sham operated group, ischemic group and IP group. Adenosine phosphate content in spinal cord of all rabbits was determined at 4 hours, 2 days and 5 days after spinal cord ischemia by using reverse phase HPLC. Results: ATP content in lumbar spinal cord of rabbits in ischemic group was markedly reduced ( P <0. 01 vs sham operated group). But in IP group, it was obviously elevated at each time point compared with ischemic group ( P <0. 01). Conclusion:IP can significantly elevated ATP content in postischemic lumbar spinal cord in rabbits. This may be one of the mechanisms that IP can protect spina1 cord from ischemic injury.