Characterization of embryo globulins encoded by the maizeGlb genes |
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Authors: | Alan L Kriz |
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Institution: | (1) Department of Agronomy, University of Illinois, 61801 Urbana, Illinois |
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Abstract: | Two of the most abundant proteins in maize embryos are saline-soluble, water-insoluble globulins. One is aM
r
63,000 protein encoded by theGlbl gene and the other is aM
r
45,000 component encoded by theGlb2 gene. Both proteins accumulate to high levels during embryo development and are rapidly degraded during the early stages
of seed germination. Amino acid composition analysis indicates that these proteins may serve as storage reserves to provide
sources of nitrogen and carbon to the germinating embryo. Amino-terminal sequence analysis of the finalGlb1 gene product, GLB1, and its immediate precursor, GLB1′, indicates that the latter is proteolytically cleaved near the amino
terminus to form GLB1. In addition to these biochemical studies, we describe the identification of a novel maize variant which
lacks the protein product of theGlb2 gene.
This contribution from the University of Illinois Agricultural Experiment Station was supported by grants from The Standard
Oil Corporation, a wholly owned subsidiary of BP America, Inc., and the U.S. Department of Agriculture (No. 88-37262-3427). |
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Keywords: | globulin embryo storage protein protein processing germination Zea mays |
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