Proteomic Analysis of Chinook Salmon (Oncorhynchus tshawytscha) Ovarian Fluid |
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| Authors: | Sheri L. Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P. Mark Lokman Neil J. Gemmell |
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| Affiliation: | 1. Allan Wilson Centre for Molecular Ecology and Evolution and Department of Anatomy, University of Otago, Dunedin, New Zealand.; 2. Department of Zoology, University of Otago, Dunedin, New Zealand.; 3. Centre for Protein Research, Department of Biochemistry, University of Otago, Dunedin, New Zealand.; Universitat de Barcelona, Spain, |
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| Abstract: | The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility, and enhancing sperm swimming speed. Here we examined if and how the proteome of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid varied among females and then sought to examine the composition of this fluid. Ovarian fluid in chinook salmon was analyzed using 1D SDS PAGE and LC-MS/MS tryptic digest screened against Mascot and Sequest databases. We found marked differences in the number and concentrations of proteins in salmon ovarian fluid across different females. A total of 174 proteins were identified in ovarian fluid, 47 of which were represented by six or more peptides, belonging to one of six Gene Ontology pathways. The response to chemical stimulus and response to hypoxia pathways were best represented, accounting for 26 of the 174 proteins. The current data set provides a resource that furthers our understanding of those factors that influence successful egg production and fertilisation in salmonids and other species. |
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