Binding of carbohydrates to solid supports: evaluation of a prototype system |
| |
Authors: | Helen J. Tweeddale Michael Batley Xing Guo Mei John W. Redmond |
| |
Affiliation: | (1) School of Chemistry, Macquarie University, 2109 North Ryde, NSW, Australia |
| |
Abstract: | Mono- and disaccharides were covalently and irreversibly bound to aminopolystyrene beads in good yield by heating in dilute aqueous solution. The degree and stability of sugar binding were determined by chemical and radiochemical methods and the accessibility of the bound sugars was demonstrated by exoglycosidase hydrolysis and by an enzyme-linked lectin-binding assay using Concanavalin A. |
| |
Keywords: | reducing sugars immobilization Amadori rearrangement aminopolystyrene immunoassay |
本文献已被 SpringerLink 等数据库收录! |