Targeted proteomics using immunoblotting technique for studying dysregulation of ion transporters in renal disorders |
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Authors: | Ecelbarger Carolyn A |
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Institution: | Department of Medicine, Georgetown University, Box 571412, Washington, DC 20057-1412, USA. ecelbarc@georgetown.edu |
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Abstract: | Renal salt and water transport physiology has benefited tremendously from the rapid advance of proteomics. Proteomics developed as a fast-throughput means of screening for global changes in proteins in a selected tissue, organ or cell type, as a logical offshoot of similar comprehensive, messenger RNA array-type technology. Targeted proteomics utilizes similar techniques but examines a predetermined set of proteins. One approach that has been rigorously employed over the last 10 years to evaluate differences in renal protein abundances due to a treatment or genotype has been parallel semiquantitative immunoblotting using antibody arrays. This approach, and newer ones on the horizon, provide a rapid global overview of regulation of the individual proteins whose integrated action determines overall renal sodium or water reabsorption. |
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