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NaCl对水稻谷氨酸合酶和谷氨酸脱氢酶的胁迫作用
引用本文:林清华,李常健,彭进,张楚富,Peng Shaobing,Bennett John. NaCl对水稻谷氨酸合酶和谷氨酸脱氢酶的胁迫作用[J]. 武汉植物学研究, 2000, 18(3): 206-210
作者姓名:林清华  李常健  彭进  张楚富  Peng Shaobing  Bennett John
作者单位:1. 武汉大学生命科学学院,武汉,430072
2. International Rice Research Institute P.O.Box 3127,1271 Makati City,Philippines
基金项目:国家自然科学基金!(编号 :3 9870 52 7),湖北省自然科学基金,国际水稻研究所资助课题
摘    要:在NaCl的胁迫下,水稻幼苗根和叶的谷氨酸合酶和谷氨酸脱氢酶的活性随着营养液中的NaCl浓度的升高而降低;游离NH4^+在叶中积累,在根中未见明显变化。与根相比,叶对NaCl的胁迫作用更为敏感。叶的NADH-GOGAT和NADH-GDH活性在NaCl胁迫降低的程度明显大于根。无论是否有NaCl存在,根的NADH-GDH活性明显高于叶。GS/GDH比值分析提示,对对照下,根中的NH4^存在,根的NA

关 键 词:谷氨酸合酶  谷氨酸脱氢酶  水稻  NaCl
收稿时间:1999-07-27
修稿时间:1999-09-15

EFFECT OF NaCl STRESS ON GLUTAMATE SYNTHASE AND GLUTAMATE DEHYDROGENASE OF RICE PLANTS
Lin Qinghua,Li Changjian,Peng Jin,Zhang Chufu,Peng Shaobing,Bennett John. EFFECT OF NaCl STRESS ON GLUTAMATE SYNTHASE AND GLUTAMATE DEHYDROGENASE OF RICE PLANTS[J]. Journal of Wuhan Botanical Research, 2000, 18(3): 206-210
Authors:Lin Qinghua  Li Changjian  Peng Jin  Zhang Chufu  Peng Shaobing  Bennett John
Affiliation:1. College of Life Sciences, Wuhan University Wuhan 430072;2. International Rice Research Institute P.O. Box 3127, 1271 Makati City, Philippines
Abstract:Glutamate synthase(GOGAT) and glutamate dehydrogenase(GDH) activity in the roots and leaves of rice seedlings were decreased under NaCl stress.NH 4 level in leaves was increased significantly due to NaCl stress,but the content of NH 4 in roots was not altered obviously,irrespective of NaCl treatment.As compared with root, leaf was more sensitive to NaCl stress.The reduction of the activity of NADH GOGAT and NADH GDH in leaves was more than that in roots.Whether NaCl was added or not to the nutrient solution,NADH GDH activity of roots was much higher than that of leaves.Analysis for GS GDH ratio showed that GS GOGAT cycle was main pathway of ammonium assimilation in the control plant roots,but under NaCl stress,GDH pathway was strengthened obviously.Irrespective of NaCl treatment,GS/GOGAT cycle was main pathway in leaves.
Keywords:Glutamate synthase   Glutamate dehydrogenase   Rice   NaCl
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