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SstI: a restriction endonuclease from Streptomyces sp. stanford.
Authors:S P Goff  A Rambach
Institution:Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, U.S.A.
Abstract:A strain of Streptomyces has been isolated which is a convenient source of a new restriction endonuclease. The enzyme has been prepared from extracts of these cells and its cleavage sites localized on phage lambda DNA. The enzyme, termed SstI, produces cohesive ends and should be useful for molecular cloning experiments.
Keywords:Enzyme purification  molecular cloning  phage lambda  cohesive ends  recombinant DNA
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