Corynebacterium aquatimens sp. nov., a lipophilic Corynebacterium isolated from blood cultures of a patient with bacteremia |
| |
Authors: | M Aravena-Román C Spröer C Siering T Inglis P Schumann A F Yassin |
| |
Affiliation: | Division of Microbiology and Infectious Diseases, Laboratory Medicine of Western Australia (PathWest), QEII MC, Locked Bag 2009, Nedlands, WA 6909, Australia. |
| |
Abstract: | An unknown lipophilic coryneform bacterium isolated from the blood cultures of a patient with bacteremia was characterized by phenotypic and molecular genetic methods. Chemical analysis revealed the presence of short chain mycolic acids consistent with the genus Corynebacterium. The DNA G+C content was 60.8mol%. Comparative 16S rRNA gene sequence analysis demonstrated that the isolate represents a new subline within the genus Corynebacterium. The closely phylogenetic relative of the unknown bacterium was found to be C. tuscaniense (97.8% sequence similarity). Partial rpoB gene sequence revealed that strain IMMIB L-2475(T) exhibited 13.5% sequence divergence with C. tuscaniense. The unknown bacterium was distinguished from C. tuscaniense by, DNA-DNA hybridization, cellular fatty acid profiles, MALDI-TOF analyses of cell extracts and biochemical tests. Based on the phylogenetic and phenotypic criteria, it is proposed that this bacterium be classified as new species, Corynebacterium aquatimens sp. nov., and is represented by strain IMMIB L-2475(T) (=DSM 45632(T)=CCUG 61574(T)). |
| |
Keywords: | Corynebacterium aquatimens sp. nov. Mycolic acid CFA 16S rRNA gene rpoB gene DNA–DNA hybridization RAPD-PCR MALDI-TOF MS |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|