|
|||||
|
|
Localization of the replicase recognition site within brome mosaic virus RNA by hybrid-arrested RNA synthesis |
|
| Authors: | Paul Ahlquist Jozef J. Bujarski Paul Kaesberg Timothy C. Hall |
| Affiliation: | (1) Biophysics Laboratory of the Graduate School, University of Wisconsin-Madison, 53706 Madison, WI, U.S.A.;(2) Department of Biochemistry, University of Wisconsin-Madison, 53706 Madison, WI, U.S.A.;(3) Department of Genetics of the College of Agricultural and Life Sciences, University of Wisconsin-Madison, 53706 Madison, WI, U.S.A. |
| Abstract: | Summary 3 terminal fragments of BMV RNA as short as 153 bases in length serve as efficient templates in vitro for BMV-specific RNA polymerase. Template activity of such fragments or of native BMV RNA is abolished when cDNA fragments as short as 39 bases are hybridized to their 3 termini. Hybridization of cDNa fragments to regions of BMV RNA 200 or more bases distal to the 3 end has no discernible effect on initiation and little effect on elongation. We conclude that BMV RNA polymerase initiates binding with an RNA template through a mechanism mediated by the tRNA-like 3 end of BMV RNA, requiring at least some of the last 39, but no more than the last 153 bases. |
| Keywords: | brome mosaic virus hybrid-arrested RNA synthesis RNA-dependent RNA polymerase viral polymerase recognition sequence |
| 本文献已被 SpringerLink 等数据库收录！ | |