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Variation in genomic alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes |
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| Authors: | Michael J. Lane P. Greg Waterbury William T. Carroll Anne M. Smardon Brian D. Faldasz Scott M. Peshick Seth Mante Clark S. Huckaby Richar E. Kouri Douglas J. Hanlon Peter J. Hahn Jane M. Scalzi John C. Hozier |
| Affiliation: | (1) Department of Medicine, State University of New York, Health Science Center at Syracuse, 50 E. Adams Street, 13210 Syracuse, NY, USA;(2) Department of Microbiology, State University of New York, Health Science Center at Syracuse, 50 E. Adams Street, 13210 Syracuse, NY, USA;(3) Genmap, Inc., 291 Whitney Avenue, 06511 New Haven, CT, USA;(4) Department of Radiology, State University of New York, Health Science Center at Syracuse, 13210 Syracuse, NY, USA;(5) Department of Medical Genetics, Florida Institute of Technology, 32901 Melbourne, FL, USA |
| Abstract: | Human DNA restriction fragments containing high numbers of Alu repeat sequences can be preferentially detected in the presence of other human DNA restriction fragments in DNA from human:rodent somatic cell hybrids when the DNA is fragmented with enzymes that cleave mammalian DNA infrequently. This ability to lower the observed human DNA complexity allowed us to develop an approach to order rapidly somatic hybrid cell lines retaining overlapping human genomic domains. The ordering process also generates a relative physical map of the human fragments detected with Alu probe DNA. This process can generate physical mapping information for human genomic domains as large as an entire chromosome (100,000 kb). The strategy is demonstrated by ordering Alu-detected NotI fragments in a panel of mouse:human hybrid cells that span the entire long arm of human chromosome 17.by L. Manuelidis |
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