Regulation of protein synthesis in rabbit reticulocyte lysates: effect of of Ca2+, Mg2+, and Mn2+ on self-phosphorylation and heterophosphorylation catalyzed by the heme-regulated and double-stranded-RNA-activated protein kinases |
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Authors: | Rajinder Singh Ranu |
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Institution: | (1) Department of Microbiology, Colorado State University, 80523 Fort Collins, Colorado, U.S.A.;(2) The Graduate Program in Cellular and Molecular Biology, Colorado State University, 80523 Fort Collins, Colorado, U.S.A. |
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Abstract: | The influence of divalent cations Mg2+, Mn2+, and Ca2+ on the cyclic-AMP-independent protein kinases of the heine-regulated and double-stranded-RNA-activated translational inhibitory protein kinases on self-phosphorylation and heterophosphorylation of the substrate (the 38 000-dalton subunit of initiation factor eIF-2) has been examined, Results show that Mg2+, Mn2+, and Ca2+ affect the activities of these enzymes in the following fashion. Mg2+ supports both self-phosphorylation and heterophosphorylation efficiently. Mn2+ on the other hand supports self-phosphorylation but to a lesser degree the heterophosphorylation, Ca2+ promotes neither self-phosphorylation nor hetero-phosphorylation. |
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