Molecular isolation and sequence determination of the cDNA for the mouse sperm-specific lactate dehydrogenase-X gene |
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| Authors: | K C Wu K Chan C Y Lee Y F Lau |
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| Affiliation: | 1. Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Liaoning 110000, Shenyang, China;2. Department of Urology, Tachengqu Hospital Affiliated Hospital of China Medical University, Tacheng 834700, Xinjiang, China;3. Department of Urology, The Fourth Affiliated Hospital of China Medical University, Liaoning 110000, Shenyang, China;4. Department of Ethnopharmacology, School of Pharmaceutical Sciences, China Medical University, Liaoning 110000, Shenyang, China;1. Department of Internal Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary;2. Department of Neurology, Bethesda Children''s Hospital, Budapest, Hungary;3. Department of Ophthalmology, Faculty of Medicine, University of Szeged, Szeged, Hungary;4. Center of Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Oslo, Norway |
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| Abstract: | Several cDNA clones for the mouse lactate dehydrogenase-X (LDH-X), a sperm-specific glycolytic enzyme, were isolated from mouse testicular cDNA libraries constructed in the bacteriophage vectors, lambda gt11 and gt10. The largest cDNA clone contains an insert of 1135 base pairs in length and an open reading frame that encodes a 332 amino acid polypeptide with a molecular weight of 35.89 kD. The deduced amino acid sequence of this protein is in close agreement with the published sequence of mouse LDH-X obtained by direct protein sequencing. Northern analysis of RNA isolated from different tissues detected a single size mRNA of 1.5 kilobases in mouse testis but not in brain or liver. The Ldh-x structural gene was estimated to be about 12 kb in size as demonstrated by Southern hybridization analysis of mouse genomic DNA using the full-length cDNA as a probe. |
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