Investigations on the interactions between naphthalimide‐based anti‐tumor drugs and human serum albumin by spectroscopic and molecular modeling methods |
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| Authors: | Huiyuan Cheng Ting Zou Yongliang Xu Ying Wang Aibin Wu Jie Dai Yezhong Zhang Yi Liu |
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| Affiliation: | 1. Department of Chemistry, College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, People's Republic of China;2. College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, Wuhan University, Wuhan, People's Republic of China |
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| Abstract: | The interactions between the three kinds of naphthalimide‐based anti‐tumor drugs (NADA, NADB, NADC) and human serum albumin (HSA) under simulated physiological conditions were investigated by fluorescence spectroscopy, circular dichroism spectroscopy and molecular modeling. The results of the fluorescence quenching spectroscopy showed that the quenching mechanisms for different drugs were static and their affinity was in a descending order of NADA > NADB > NADC. The relative thermodynamic parameters indicated that hydrophobic force was the predominant intermolecular force in the binding of NAD to HSA, while van der Waals interactions and hydrogen bonds could not be ignored. The results of site marker competitive experiment confirmed that the binding site of HSA primarily took place in site I. Furthermore, the molecular modeling study was consistent with these results. The study of circular dichroism spectra demonstrated that the presence of NADs decreased the α‐helical content of HSA and induced the change of the secondary structure of HSA. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | human serum albumin naphthalimide‐based anti‐tumor drugs fluorescence quenching circular dichroism molecular modeling |
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