A ‘turn‐on’ fluorescent chemosensor for quantification of serum albumin in aqueous solution at neutral pH |
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Authors: | Yuanyuan Li Kai Li Juan He |
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Affiliation: | 1. School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, People's Republic of China;2. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, People's Republic of China |
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Abstract: | A fluorescent chemosensor 1 (4‐diethylamino‐2′‐hydroxychalcone) for detecting serum albumin with long‐wavelength emission, good selectivity and facile synthesis was reported. Upon the addition of bovine serum albumin (BSA) to an aqueous solution of 1 at neutral pH, a ‘turn‐on’ fluorescence response was observed at 596 nm based on a hydrophobic binding mode between 1 and BSA. A linear range of 0.10–1.00 mg/mL and a detection limit of 9.1 µg/mL for BSA were obtained, respectively. Moreover, 1 was successfully applied to detect BSA in real bovine serum samples with satisfied recovery and accuracy, which suggested that 1 could serve as a valid and effective fluorescent chemosensor for quantification of BSA. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | serum albumin fluorescence turn‐on chemosensor long‐wavelength emission |
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