A water‐soluble fluorescent pH probe based on perylene dyes and its application to cell imaging |
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| Authors: | Yongshan Ma Fengxia Zhang Jinfeng Zhang Tianyi Jiang Xuemei Li Junsen Wu Huixue Ren |
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| Institution: | 1. School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, People's Republic of China;2. Shandong Provincial Key Laboratory of Metrology and Measurement, Shandong Institute of Metrology, Shandong Social Justice Institute of Metrology, Jinan, People's Republic of China |
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| Abstract: | A fluorescent pH probe, N,N′‐bi( l ‐phenylalanine amine)‐perylene‐3,4;9,10‐dicarboxylic diimide (PDCDA) was synthesized and used for pH sensing in living cells. A significant fluorescence intensity change was observed over a pH range from 7.0 to 4.0. Electrostatic potential maps (MEP) suggested that the electronic repulsion between PDCDAs was increased by the high negative electrostatic potential which resulted in a high water solubility of PDCDA. PDCDA was successfully applied as a high‐performance fluorochrome for living HeLa cell imaging. The results demonstrate that the probe PDCDA is a good candidate for monitoring pH fluctuations in living cells with good water solubility, low cytotoxicity, high fluorescence quantum yield and photostability. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | living cell staining water‐soluble perylene derivative fluorescence pH probe |
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