A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters |
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Authors: | Morteza Hosseini Fatemeh Mehrabi Mohammad Reza Ganjali Parviz Norouzi |
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Affiliation: | 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran;2. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran;3. Biosensor Research Center, Endocrinology & Metabolism Molecular‐Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran |
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Abstract: | A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd. |
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Keywords: | oxytetracycline silver nanoclusters fluorescence quenching aptamer nanobiosensor |
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