Glucosylation of Salicyl Alcohol by Gardenia jasminoides Cell Cultures |
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Authors: | Mizukami Hajime; Terao Toshimitsu; Amano Akemi; Ohashi Hiromu |
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Affiliation: | Faculty of Pharmaceutical Sciences, Nagasaki University Bunkyo-machi, Nagasaki 852, Japan |
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Abstract: | Cultured cells of Gardenia jasminoides produced both salicinand isosalicin from exogenously supplied salicyl alcohol. Theglucosylation activity of the cells was highest in the exponentialphase of growth and ca. 70% of the added substrate was convertedto the glucosides within 4 days. The rate of glucosylation wasalso dependent on the medium composition such as auxin and sucroseconcentrations. The ratio of salicin to isosalicin formed fromsalicyl alcohol was influenced by the growth stage of the culturedcells. Salicin was converted to isosalicin when exogenouslyadded to the culture. (Received October 11, 1985; Accepted March 10, 1986) |
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