Stereoselective enzymatic synthesis of an aspartame precursor of N-CBZ-
-Asp-
-PheOMe |
| |
| Authors: | Geun Hee Shin Chulyoung Kim Hyun Jung Kim Chul Soo Shin |
| |
| Institution: | Department of Biotechnology, College of Engineering, Bioproducts Research Center, Seodaemun-gu, Yonsei University, Seoul 120-749, South Korea |
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| Abstract: | A stereoselective protease produced by Bacillus amyloliquefaciens KCCM 12091 was isolated. The enzyme catalyzed the synthesis of N-CBZ-
-Asp-PheOMe from N-CBZ-
-Asp and
-PheOMe, but not N-CBZ-
-Asp-
-PheOMe from N-CBZ-
-Asp and
-PheOMe. More than 50% of added
-PheOMe was consumed when eutectic mixtures of N-CBZ-
-Asp, racemic
- and
-PheOMe were used for synthesis of an aspartame precursor of N-CBZ-
-Asp-
-PheOMe.
-PheOMe was not involved in the reaction and did not affect synthesis of N-CBZ-
-Asp-
-PheOMe. |
| |
| Keywords: | Aspartame precursor Stereoselective protease Eutectic substrate mixture |
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