An efficient method for purification of PCR products for sequencing |
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Authors: | Ma Hao Difazio Stephen |
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Affiliation: | Department of Biology, West Virginia University, Morgantown, WV 26506, USA. hao.ma@mail.wvu.edu |
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Abstract: | A high-throughput DNA sequencing method that generated high quality data was developed. A frame fashioned from a standard agarose gel combined with 0.1%-0.2% low-melting point (LMP) agarose gel was used to isolate the PCR product of interest. Collected PCR products were centrifuged without any reagents and the supernatants were directly used for a sequencing reaction. This method is simple and labor efficient, provides high quality sequences at a low cost, and bypasses problems with impure PCR products. This technique has been used for single nucleotide polymorphism (SNP) discovery in Populus angustifolia trees. |
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