Characterization of NADH: nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae) |
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Authors: | Iwamoto Koji Shiraiwa Yoshihiro |
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Institution: | (1) Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan, |
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Abstract: | Nitrate reductase was purified from and characterized in a bloom-forming unicellular calcifying alga, Emiliania huxleyi (Haptophyceae). The molecular masses of the native form and the subunit were 514 and 85 kDa, respectively, showing that the enzyme is a hexamer composed of 6 homologous subunits. The Km values for NADH and NO3- were 40 microM and 104 microM, respectively. Activity of the reduction of nitrate was very high with reduced methylviologen and NADH, but no activity was observed with NADPH or reduced flavin mononucleotide; oxidation of NADH was very high with cytochrome c but did not occur with ferricyanide. These results indicate that Emiliania nitrate reductase is NADH-specific (EC 1.6.6.1), and that among algae and plants its subunit structure and kinetic properties are unique. |
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Keywords: | coccolithophorid Emiliania huxleyi enzyme purification nitrate reductase nitrate utilization |
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