Respiratory stimulation and generation of superoxide radicals in Pseudomonas aeruginosa by fungal naphthoquinones |
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Authors: | H Haraguchi Kazumi Yokoyama Shingo Oike Masakazu Ito Hiroshi Nozaki |
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Institution: | (1) Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama 729-02, Japan Tel. +81-849-36-2111; Fax +81-849-36-2023, JP;(2) Faculty of Science, Okayama University of Science, Ridai-cho, Okayama 700, Japan, JP |
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Abstract: | The mechanism of action of antimicrobial naphthoquinones from the fungus Fusarium was studied by using Pseudomonas aeruginosa. Bostricoidin, methyl ether fusarubin, and fusarubin stimulated the oxygen consumption of bacterial cells and induced cyanide-insensitive
oxygen consumption. These activities of the tested compounds were also observed in bacterial membrane preparations in a dose-dependent
manner. Naphthoquinones stimulated the generation of superoxide anion and hydrogen peroxide. The naphthoquinone effectively
acted as the electron acceptors for bacterial diaphorase, which could explain the antibacterial activity of Fusarium naphthoquinones since electron acceptors lead to the stimulation of respiratory activity and the generation of oxygen radical
species.
Received: 12 July 1996 / Accepted: 31 October 1996 |
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Keywords: | Fungal naphthoquinones Respiratory burst Superoxide anion Electron acceptor Antibacterial activity |
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