Ontogenetic Changes in Glial Fibrillary Acid Protein
Phosphorylation,Glutamate Uptake and Glutamine Synthetase Activity in Olfactory Bulb of Rats |
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Authors: | Cíntia?Eickhoff?Battú Gra?a?F R S?Godinho Ana?Paula?Thomazi Lúcia?M V?de ?Almeida Carlos?Alberto?Gon?alves Trícia?Kommers Email author" target="_blank">Susana?T?WofchukEmail author |
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Institution: | (1) Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, UFRGS, 90035-003 Porto Alegre, RS, Brasil;(2) Centro Universitário Lasalle, UNILASALLE, Victor Barreto 2288, 92010-000 Canoas, RS, Brasil |
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Abstract: | Phosphorylation of the glial fibrillary acidic protein (GFAP) in hippocampal and cerebellar slices from immature rats is stimulated
by glutamate. This effect occurs via a group II metabotropic glutamate receptor in the hippocampus and an NMDA ionotropic
receptor in the cerebellum. We investigated the glutamate modulation of GFAP phosphorylation in the olfactory bulb slices
of Wistar rats of different ages (post-natal day 15 = P15, post-natal day 21 = P21 and post-natal day 60 = P60). Our results
showed that glutamate stimulates GFAP phosphorylation in young animals and this is mediated by NMDA receptors. We also observed
a decrease in glutamate uptake at P60 compared to P15, a finding similar to that found in the hippocampus. The activity of
glutamine synthetase was elevated after birth, but was found to decrease with development from P21 to P60. Together, these
data confirm the importance of glutamatergic transmission in the olfactory bulb, its developmental regulation in this brain
structure and extends the concept of glial involvement in glutamatergic neuron-glial communication. |
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Keywords: | Brain development GFAP glutamate uptake glutamine synthetase NMDA olfactory bulb |
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