Application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product) |
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Authors: | Kammoun Radhouane Naili Belgacem Bejar Samir |
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Affiliation: | Centre de Biotechnologie de Sfax, Laboratoire d'Enzymes et Métabolites des Procaryotes, Route de sidi Mansour km 4, BP"K" 3038 Sfax, Tunisia. radhouan.kammoun@cbs.rnrt.tn |
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Abstract: | The production optimization of alpha-amylase (E.C.3.2.1.1) from Aspergillus oryzae CBS 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. The optimisation of temperature, agitation and inoculum size was attempted using a Box-Behnken design under the response surface methodology. The screening of nineteen nutrients for their influence on alpha-amylase production was achieved using a Plackett-Burman design. KH(2)PO(4), urea, glycerol, (NH(4))(2)SO(4), CoCl(2), casein hydrolysate, soybean meal hydrolysate, MgSO(4) were selected based on their positive influence on enzyme formation. The optimized nutrients concentration was obtained using a Taguchi experimental design and the analysis of the data predicts a theoretical increase in the alpha-amylase expression of 73.2% (from 40.1 to 151.1 U/ml). These conditions were validated experimentally and revealed an enhanced alpha-amylase yield of 72.7%. |
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Keywords: | Aspergillus oryzae α -Amylase Box– Behnken design Plackett– Burman design Taguchi design |
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