Isolation of Intracytoplasmic Membrane from the Methanotrophic Bacterium Methylomicrobium album BG8 |
| |
| Authors: | Christine A Brantner Lorie A Buchholz Charles C Remsen Mary Lynne Perille Collins |
| |
| Institution: | (1) Department of Biological Sciences and Great Lakes WATER Institute, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA , US |
| |
| Abstract: | Methane-oxidizing bacteria, including Methylomicrobium album BG8, form an intracytoplasmic membrane in addition to the cytoplasmic and outer membranes of the cell envelope. Techniques
to isolate the intracytoplasmic membrane of M. album BG8 were developed. An intracytoplasmic membrane fraction was separated from a cell envelope fraction on the basis of sedimentation
velocity in sucrose density gradients. Proteins associated with the particulate methane monooxygenase were found in both membrane
fractions.
Received: 27 July 1999 / Accepted: 30 August 1999 |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
