番茄灰霉病拮抗菌株初步鉴定及通过导入几丁质酶基因提高其生防效果

实验室保存菌Fh对番茄灰霉病有明显的抑制作用,通过形态特征、生理生化特征进行初步鉴定归属于芽孢杆菌属（Bacillus circulans）。从质粒pUC1965中得到含有几丁质酶基因的6.5kb DNA片段，将该基因片段与大肠杆菌 枯草芽孢杆菌穿梭质粒pBE2连接,获得重组质粒pBE2-chib。将重组质粒转入芽孢杆菌Fh中获得工程菌株Fh-chib。几丁质酶基因的PCR检测和几丁质平板实验表明几丁质酶基因被成功转入，工程菌株Fh-chib的原始粗酶液几丁质酶活为4.06U/ml。与野生菌相比，Fh-chib工程菌株对番茄灰霉病（Botrytis cinerea）抑菌效果提高34.46%。

The Elementary Identification of Antagonistic Bacteria Against Botrytis cinerea and Improving Biocontrol Effect Through Genetic Modification with Chitinase Gene

Fh antagonist to Botrytis cinerea was preserved in laboratory.Through a series of morphological and biochemical characteristics,Fh belongs to Bacillus.In order to enhance the inhibitory effect of this strain,a 6.4 kb DNA fragment containing chitinase gene from pUC1965 was inserted into vector pBE2 to construct recombination plasmids called pBE2-chib.A new strain named Fh-chib was formed by transferring the recombination plasmids into Bacillus Fh.The PCR identification of chitinase gene and chitin plate cult...