Perimicrovillar membranes promote hemozoin formation into Rhodnius prolixus midgut |
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| Authors: | Silva José R Mury Flávia Borges Oliveira Marcus F Oliveira Pedro L Silva Carlos P Dansa-Petretski Marílvia |
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| Institution: | 1. Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ 28013-612, Brazil;2. Instituto de Bioquímica Médica, Programa de Biotecnologia e Biologia Molecular, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ 21941-590, Brazil;1. Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina;2. Departamento de Bioquímica Clínica, Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina;3. Laboratorio de Análisis Clínicos Especializados (LACE), Córdoba, Argentina;1. Laboratory of Genetics and Functional Genomics, Regional Center for Genomic Studies, Faculty of Exact Sciences, National University of La Plata, Bvd 120 y 62, 1900, La Plata, Buenos Aires, Argentina;2. Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, bloco D, Prédio do CCS, Ilha do Fundão, Rio de Janeiro, 21941-902, Brazil;3. Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Zoological Institute, K.U. Leuven, Leuven, Belgium;4. Centre of Experimental and Applied Endocrinology, National University of La Plata, School of Medicine, 60 Street y 120, 1900, La Plata, Buenos Aires, Argentina;1. Department of Biochemistry, Universidade Federal de São Paulo, Escola Paulista de Medicina, 04044-020 São Paulo, SP, Brazil;2. Department of Biochemistry, Instituto de Química, Universidade Federal do Rio de Janeiro, 21941-909 Rio de Janeiro, RJ, Brazil;3. Department of Parasitology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil;4. Centro de Pesquisas René Rachou, FIOCRUZ, 30190-002 Belo Horizonte, MG, Brazil;1. Departamento de Biodiversidad y Biología Experimental (DBBE), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Argentina;2. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), CONICET-UBA, Argentina |
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| Abstract: | Rhodnius prolixus is a hematophagous insect that ingests large quantities of blood in each blood-feeding session. This ingested blood provides important nutrients to sustain the insect's oogenesis and metabolic pathways. During the digestive process, however, huge amounts of heme are generated as a consequence of the hemoglobin breakdown. Heme is an extremely dangerous molecule, since it can generate reactive oxygen species in the presence of oxygen that impair the normal metabolism of the insect. Part of the hemoglobin-derived heme can associate with the perimicrovillar membranes (PMM) in the gut lumen of R. prolixus; in this study we demonstrate the participation of the PMM in a heme detoxification process. These membranes were able to successfully induce heme aggregation into hemozoin (Hz). Heme aggregation was not dependent on the erythrocyte membranes, since the contribution of these membranes to the process was negligible, demonstrating that the ability to induce heme aggregation is a feature of the PMM, possibly representing a pre-adaptation of the hemipterans to feeding on blood. |
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