Targeted mapping approaches to identify DNA markers linked to the Rfp1 restorer gene for the ‘Polima’ CMS of canola (Brassica napus L.) |
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Authors: | M Jean G G Brown B S Landry |
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Institution: | (1) McGill University, Biology Department, 1205 ave. Doctor Penfield, Montréal, Québec, Canada, H3A 1B1, CA;(2) DNA LandMarks, P.O. Box 6, St-Jean-sur-Richelieu, Québec, Canada, J3B 6Z1, CA |
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Abstract: | We have used two targeting approaches pairs of nearly isogenic lines (NILs) and bulked segregant analysis] to identify DNA
markers linked to the Rfp1 restorer gene for the pol CMS of canola (Brassica napus L.). We were able to target the Rfp1 locus as efficiently by comparing NILs as by bulked segregant analysis, and it was demonstrated in this instance that double-screening
strategies could significantly improve the overall targeting efficiency. The chance occurrence of shared homozygosity at specific
unlinked chromosomal regions in the bulks was found to limit the efficiency of bulked segregant analysis, while the efficiency
of NIL comparison was limited by residual DNA from the donor cultivar at scattered sites throughout the genome of the NILs.
Received: 6 June 1997 / Accepted: 12 February 1998 |
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Keywords: | Targeted mapping RFLP RAPD Brassica napus Polima CMS Nearly isogenic line Bulked segregant analysis |
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