Effects of antibodies to the filamentous haemagglutinin and to the pertussis toxin of Bordetella pertussis on adherence and toxic effects to 3T3 cells |
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Authors: | Sylvie Lenin Jean Michel Alonso Colette Brezin Murielle Rocancourt Olivier Poupel |
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Affiliation: | Bacterial Ecology Unit, Department of Bacteriology and Mycology, Institut Pasteur, 25–28 rue du Dr. Roux, 75724 Paris Cedex 15, France;Replication Physiology Unit, Department of Bacteriology and Mycology, Institut Pasteur, 25–28 rue du Dr. Roux, 75724 Paris Cedex 15, France |
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Abstract: | Abstract Adherence of B. pertussis to NIH3T3 mouse fibroblasts was efficiently inhibited by a mouse immune serum reacting specifically with the filamentous haemagglutinin (FHA), whereas a mouse immune serum reacting specifically with the pertussis toxin (Ptx) produced partial inhibition only significant after 3 h infection. Protection against cytopathic effects on infected 3T3 cells with anti-FHA antibodies was at least as effective (83.3%± 7.5) as with anti-Ptx antibodies (75%± 4). This suggests that adherence of B. pertussis to eukaryotic receptors is a primary mechanism determining both bacterial proliferation and toxic effects in susceptible cells, and that prevention of B. pertussis attachment to cell receptors might be sufficient to protect against both infectious and toxic processes in whooping cough. |
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Keywords: | Bordetella pertussis adherence pertussis toxin haemagglutinin effects of antibodies |
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