首页 | 本学科首页   官方微博 | 高级检索  
     


NaCl-dependent formation of the highly crystalline phase in sufficiently hydrated dimyristoylphosphatidylglycerol bilayers
Authors:Masanao Kinoshita  Satoru Kato
Affiliation:a Department of Physics, School of Science and Technology, Kwansei-Gakuin University, 2-1, Gakuen, Sanda, Hyogo, 669-1337, Japan
b Biophysics Laboratory, Graduate School of Engineering, Gunma University, 4-2, Aramaki, Maebashi, Gunma, 371-8510, Japan
Abstract:We investigated the low-temperature phase behavior of dimyristoylphosphatidylglycerol (DMPG) bilayers in the presence of high concentration of NaCl (≥100 mM). Differential scanning calorimetry showed that the highly crystalline (HC) phase grew after an initial delay period when DMPG bilayers were sufficiently hydrated and incubated at 1 °C in the presence of more than 100 mM NaCl. The HC phase formation reached a plateau, the level of which depended on NaCl concentration; all the lipids were unable to be in the HC phase at the plateau stage without a quite high concentration of NaCl. Since electron microscopic observations suggested that the HC phase formed coexists with the precursor phases in a closed vesicle, elastic constrain and/or shortage of free sodium ions in the inside of the closed vesicle may prevent the complete transition into the HC phase.
Keywords:DMPG   Highly crystalline phase formation   Kinetics   DSC   Electron microscopy   X-ray diffraction
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号