A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere |
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| Authors: | Michael Tsabar Julian Haase Benjamin Harrison Chloe E. Snider Brittany Eldridge Lila Kaminsky Rebecca M. Hine James E. Haber Kerry Bloom |
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| Affiliation: | 1Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts, United States of America;2Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina;Duke University, UNITED STATES |
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| Abstract: | Transcriptional inactivation of the budding yeast centromere has been a widely used tool in studies of chromosome segregation and aneuploidy. In haploid cells when an essential chromosome contains a single conditionally inactivated centromere (GAL-CEN), cell growth rate is slowed and segregation fidelity is reduced; but colony formation is nearly 100%. Pedigree analysis revealed that only 30% of the time both mother and daughter cell inherit the GAL-CEN chromosome. The reduced segregation capacity of the GAL-CEN chromosome is further compromised upon reduction of pericentric cohesin (mcm21∆), as reflected in a further diminishment of the Mif2 kinetochore protein at GAL-CEN. By redistributing cohesin from the nucleolus to the pericentromere (by deleting SIR2), there is increased presence of the kinetochore protein Mif2 at GAL-CEN and restoration of cell viability. These studies identify the ability of cohesin to promote chromosome segregation via kinetochore assembly, in a situation where the centromere has been severely compromised. |
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