Highly Sensitive Detection of Melamine Using a One-Step Sample Treatment Combined with a Portable Ag Nanostructure Array SERS Sensor |
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| Authors: | Jie Cheng Xiao-Ou Su Yue Yao Caiqin Han Shi Wang Yiping Zhao |
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| Institution: | 1Institute of Quality Standards and Testing Technologies for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, China;2School of Physics and Electronic Engineering, Jiangsu Normal University, Jiangsu, China;3Department of Physics and Astronomy, University of Georgia, Athens, United States of America;University of Gdansk, POLAND |
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| Abstract: | There is an urgent need for rapid and reliable methods able to detect melamine in animal feed. In this study, a quick, simple, and sensitive method for the determination of melamine content in animal feed was developed using surface-enhanced Raman spectroscopy on fabricated Ag nanorod (AgNR) array substrates with a one-step sample extraction procedure. The AgNR array substrates washed by HNO3 solvent (10−7 M) and methanol and showed the good stability within 6 months. The Raman shift at △ν = 682 cm−1 was used as the characteristic melamine peak in the calculations. Sufficient linearity was obtained in the 2–200 μg·g−1 range (R2 = 0.926). The limits of detection and quantification were 0.9 and 2 μg·g−1, respectively. The recovery rates were 89.7–93.3%, with coefficients of variation below 2.02%. The method showed good accuracy compared with the tradition GC-MS analysis. This new protocol only need 2 min to fininsh the detection which could be developed for rapid onsite screening of melamine contamination in quality control and market surveillance applications. |
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