Nitric Oxide Interferes with Islet Cell Zinc Homeostasis |
| |
Authors: | Ulrike Tartler, Klaus-D. Kr ncke, Klaus L. Meyer, Christoph V. Suschek,Victoria Kolb-Bachofen |
| |
Affiliation: | Research Group Immunobiology, Medical Department, Heinrich-Heine-University, Düsseldorf, Germany. |
| |
Abstract: | Zinc is crucial for the biosynthesis, storage, and secretion of insulin in pancreatic islet cells. We have previously presented evidence that NO interferes with cellular Zn(2+) homeostasis and we therefore investigated the influence of chronic NO exposure on the labile islet cell Zn(2+) content. A strong fluorescence activity in a large islet cell subpopulation was found after staining with the Zn(2+)-specific fluorophore Zinquin. Culture for 24 h in the presence of nontoxic concentrations of the slow-releasing NO donor DETA/NO resulted in a significantly reduced Zn(2+)-dependent fluorescence. This appears to be islet specific as in endothelial cells DETA/NO exposure enhanced the Zn(2+)-dependent fluorescence activity in a concentration-dependent manner. These results suggest that NO interferes with cellular Zn(2+) homeostasis, which in islet cells is crucial for proper hormone delivery and thus special cell function. |
| |
Keywords: | diabetes endothelial cells islet cells nitric oxide zinc |
本文献已被 ScienceDirect 等数据库收录! |
|