A method for increasing cellulase production by Trichoderma viride

A doubling of cellulase production by Trichoderma viride (QM9414) is possible by increasing the cellulose concentration in the medium from 0.75 to 2%, increasing the nitrogen concentration, and controlling pH during growth. A four-to fivefold increase in β -glucosidase is found with the higher cellulose concentration. Culture filtrates from 2% cellulose cultures can reduce the hydrolysis time in a practical saccharification to one-half that required by culture filtrates from 0.75% cellulose cultures.