Mating reaction in Saccharomyces cerevisiae |
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Authors: | Chikashi Shimoda Naohiko Yanagishima Akira Sakurai Saburo Tamura |
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Institution: | (1) Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, 558 Osaka, Japan;(2) Department of Biology, Faculty of Science, Nagoya University, 464 Nagoya, Japan;(3) The Institute of Physical and Chemical Research, 351 Saitama, Japan |
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Abstract: | A diffusible sex-specific substance called substance-I ( S-I) was isolated from culture filtrate of type strains of the yeast Saccharomyces cerevisiae. The isolated S-I, an oligopeptide, induced sexual cell agglutinability in inducible a type strains and enhanced the agglutinability in constitutive a type strains. The induction of sexual agglutinability was detected in 30 min and reached maximum in 90 min, when 0.2 g/ml of S-I was added to inducible a type cells. The a type-specific factor responsible for sexual cell agglutination, called a type agglutination factor (aAF), was shown to be produced during the induction or the enhancement of agglutinability of a type cells by S-I. The aAF produced in response to S-I was not different in the susceptibility to proteolytic enzymes and disulfide-cleaving agents from those produced constitutively in the absence of S-I. |
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Keywords: | Saccharomyces cerevisiae Mating reaction Sexual cell agglutination substance-I" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0"> substance-I Agglutination factor |
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