Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil |
| |
Authors: | Martins Luisa Caricio Corvelo Tereza Cristina de Oliveira Demachki Samia Araujo Marialva T F Assumpção Mônica Baraúna Vilar Simone Cristina Araujo Jucá Freitas Felipe Bonfim Barbosa Hivana Patricia Melo Fecury Amanda Alves do Amaral Renata Kelly Costa Dos Santos Sidney Emanuel Batista |
| |
Affiliation: | Laboratório de Imunogenética, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. lu-caricio@bol.com.br |
| |
Abstract: | We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|