综述与专论: 基因编辑猪中条件性基因修饰系统的研究进展

条件性基因修饰能够使基因修饰事件在特定组织器官的特定时间内发生，可通过在动物中引入条件性基因缺失系统和条件性基因过表达系统来实现，在小鼠中已被广泛的应用于基因功能的研究。基因修饰猪在农业领域和生物学领域中均具有重要的应用价值。在生物医药领域，由于猪的体型、器官大小、生理、代谢以及寿命与人类具有很多的相似性，基因修饰猪既可用于基因功能的基础研究，也可用作人类疾病模型、异种移植的器官来源以及人类功能蛋白生物反应器。由于最近几年基因编辑技术的进展，使在猪体内引入基因表达条件性系统设想成为可能，构建基因表达条件性调控工具猪模型，将极大地推动其研究进展。本文综述了利用基因编辑技术，构建条件性修饰系统及基因修饰猪模型取得的进展。

Review: Progress on Application of Conditional System in Gene-editing Pigs

Conditional gene-editing system can limit gene modification occur in specific tissues or organs at a specific time, which can be realized by introducing conditional gene deletion system and conditional gene overexpression system into animals, and has been widely used in the study of gene function in mice. Gene-editing pigs have important application value in agriculture and biology. In the field of biological medicine, because of the similarities of body, organ size, physiology, metabolism, and life with humans, genetically-modified pigs can be used for the basic research of gene function, and as a model of human disease, xenotransplantation supply and human functional protein bioreactor. Benefit from the development of gene editing technology in recent years, it is possible to introduce the conditional gene expression system in pigs, and conditional gene expression pig model will greatly promote the research progress. In this paper, we review the progress in the construction of conditional modification systems and genetically-modified pig models by using gene editing techniques.