贵州部分洞穴裸灶螽的鉴定及其遗传多样性分析

贵州是中国具有丰富喀斯特洞穴生态系统的省份之一,裸灶螽作为洞穴中的优势物种又为生态环境指示种,其遗传多样性能够反应洞穴生物多样性.为评估洞穴开发对洞穴生物多样性的影响,本研究通过形态鉴定和分子辅助鉴定的方式对来自贵州省毕节、黔西南、铜仁和黔南4个地区8个洞穴97号裸灶螽标本进行鉴定,并对其遗传多样性进行分析.结果共鉴定...

Identification and genetic diversity analysis of Tachycines ( Gymnaeta )in some caves of Guizhou Province

Guizhou Province is one of the provinces with rich karst cave ecosystem in China. As indicator species of ecological environment, the species of Rhaphidophoridae are dominant cave insects. As the dominant species in the cave and the indicator species of ecological environment, the genetic diversity of Gymnaeta can reflect the biodiversity of caves. In order to evaluate the impact of cave development on the biodiversity of cave, the present study identified 97 specimens of Tachycines ( Gymnaeta )from eight caves in Bijie, Qianxinan, Tongren and Qiannan by morphological identification and molecular assisted identification, and analyzed their genetic diversity. Eight species of Tachycines ( Gymnaeta )and three undetermined species with ambiguous morphological identification were identified, including Tachycines ( Gymnaeta ) fallax and Tachycines ( Gymnaeta ) cavernus, which were first reported in Guizhou. By using mitochondrial COI gene to construct the phylogenetic tree of Tachycines ( Gymnaeta ), the indeterminate species were identified. It is suggested that there may be complex species among Tachycines ( Gymnaeta ) cavernus, Tachycines ( Gymnaeta ) solidus and Tachycines ( Gymnaeta ) borutzkyi. Small cave space and low relative humidity, human disturbance and serious cave development will reduce the population and genetic diversity of Tachycines ( Gymnaeta ).Therefore, it is suggested to establish cave species gene bank for caves with large cave space and rich species, so as to develop tourism resources scientifically and promote ecological science and technology tourism.