Yeast species recognition from gene sequence analyses and other molecular methods |
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Authors: | Cletus P Kurtzman |
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Institution: | (1) Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA |
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Abstract: | This review discusses DNA-based methods used for identification of yeasts. Nuclear DNA reassociation was the first quantitative
molecular method employed for recognition of yeast species and has provided a baseline for interpretation of other molecular
comparisons. Among these, gene sequencing is the most definitive method, with ribosomal RNA gene sequences providing the preponderance
of available data. Multigene analyses that include the sequences of protein encoding genes are being increasingly developed
to provide a more definitive resolution of species. A number of rapid identification methods, such as denaturing gradient
gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), and flow cytometry, which are based on species-specific
gene sequences, are available for use in diagnostic laboratories. |
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Keywords: | Gene sequences Molecular identification Phylogenetic species Rapid species identification Yeasts |
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