| Abstract: | In the post-genome era, technologies are becoming availablethat allow the profiling of tissues and cell populations atmultiple levels including genomic (DNA and RNA), proteomic (proteinsand peptides) and post-proteomic (eg metabolomic). Operomicsrefers to the molecular analysis of tissues and cells at thethree levels that are connected through the coding process —namely, DNA, RNA and protein. The premise is that no one levelor type of analysis fully captures gene expression and thatfunctional changes at the proteome level cannot be simply predictedfrom analyses at the DNA or RNA levels. An important determinantthat weakens a direct link between RNA and protein levels istranslational control that differentially regulates mRNA translation.In this paper, the approaches for genomic and proteomic profilingand the contribution of translational control are reviewed. |
