DNA synthesis in Escherichia coli during a nutritional shift-up |
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| Authors: | Arieh Zaritsky and Shmuel Zabrovitz |
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| Institution: | (1) Department of Biology, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva, Israel;(2) Present address: Department of Microbiology and Immunology, University of California, 94720 Berkeley, CA, USA |
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| Abstract: | Summary DNA synthesis in a thymine-requiring Escherichia coli K12 strain was studied by exploiting deoxyguanosine, so simulating the behaviour of Thy+ strains. DNA synthesis is inhibited during the first 25 min after a nutritional shift-up. The new DNA/mass is lower than that predicted by current models for initiation control.Dedicated to the memory of Shmuel Zabrovitz, whose high spirits and good humor enabled him to complete the work while struggling with his lethal diseaseDeceased |
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