| 中德生物STR荧光ZDgenetype 9+1基因分型试剂盒应用结果分析 |
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| 引用本文： | 周文斌,刘未斌,裴君,杨辉,熊勇华.中德生物STR荧光ZDgenetype 9+1基因分型试剂盒应用结果分析[J].生物技术,2006,16(1):45-49. |
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| 作者姓名： | 周文斌 刘未斌 裴君 杨辉 熊勇华 |
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| 作者单位： | 1. 江西省高级人民法院司法技术处,江西,南昌,330046
2. 江西中德生物工程有限公司,江西,南昌,330029 |
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| 基金项目： | 国家科技型中小企业创新基金资助项目(03C23213600390) |
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| 摘 要： | 目的:确证中德生物STR荧光ZDgenetype 9 1基因分型试剂盒应用于个体基因分型的准确性和重复性。方法:应用中德生物ZDgenetype 9 1 STR荧光基因分型试剂盒扩增100例无关个体样本,建立扩增产物在ABI 310遗传分析仪上基因分型的方法,相同样本扩增结果与AmpFLSTR Identifiler基因分型结果进行比较。结果:ZDgenetype 9 1荧光基因分型试剂盒对100例无关个体血样进行复合扩增并实现了在ABI 310遗传分析仪上进行基因分型,其结果与AmpFLSTR Identifile扩增相同样本的基因分型结果吻合率为100%。结论:中德生物ZDgenetype 9 1 STR荧光基因分型试剂盒适用于法庭科学日常办案和科研使用。
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| 关 键 词： | 短串联重复序列(STR) ZDgenetype9 1试剂盒 PCR 基因分型 |
| 文章编号： | 1004-311X(2006)01-0045-05 |
| 修稿时间： | 2005年9月5日 |
Application of ZDgenetype 9 + 1 kit for Genotyping in Forensic Science |
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| ZHOU Wen-bin,LIU Wei-bin,PEI Jun,YANG Hui,XIONG Yong-hua.Application of ZDgenetype 9 + 1 kit for Genotyping in Forensic Science[J].Biotechnology,2006,16(1):45-49. |
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| Authors: | ZHOU Wen-bin LIU Wei-bin PEI Jun YANG Hui XIONG Yong-hua |
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| Abstract: | Objective:To test ZDgenetype kit'DNA sample amplification result in forensic areas: veracity and repeatability.Methods: Used ZDgenetype 9 1 kit and AmpFLSTR Identifiler kit amplification 100 samples,then put the PCR product in ABI3100 for polyacrylamide gel electrophoresis.Analyzed the results by Genescan and Genotyper(softwares were developed by appliedbiosystems US) and compared analysis results which get from ABI310).Results: The genotyping result which came from ZDgenetype kit and AmpFLSTR Identifiler kit was highly consistency.Conclusions:The genotyping result of ZDgenetype kit was coincident and repeatability,so ZDgenetype 9 1 kit which developed by Jiangxi Zodogenes Biotechnology Co,Ltd was suitable to case and research in forensic areas. |
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| Keywords: | STR(short tandem repeat) ZDgenetype kit PCR genotype |
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