Affinity labeling of phosphoenolpyruvate carboxykinase with 1, 5-I-AEDANS. |
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| Authors: | R Silverstein A B Rawitch D A Grainger |
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| Affiliation: | 1. Division of Biochemistry, Physiology, and Pharmacology University of South Dakota School of Medicine, Vermillion, South Dakota 57069, USA |
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| Abstract: | The concentrations of zinc thionein and cytosolic zinc in rat liver were examined in male rats five days after bilateral adrenalectomy. Zinc in metallothionein increased 10 fold, as compared with control animals. Cytosolic zinc increased 79% as compared with controls. 65% of this increase could be accounted for bound to metallothionein. Sham operated animals after five days showed a 4 fold increase in hepatic zinc thionein and a 23% increase in cytosolic zinc, 71% of this increase being bound to metallothionein. Adrenalectomized rats, maintained on daily injections of corticosterone (4mg/100g b.w.), exhibited the same levels of zinc thionein and cytosolic zinc as adrenalectomized rats receiving no treatment. Adrenalectomized rats, maintained on daily injections of aldosterone (5μg/100g b.w.), exhibited the same levels of zinc thionein as the sham operated rats, but the cytosolic zinc remained elevated at the level found in adrenalectomized rats receiving no treatment. These results indicate that there is adrenal involvement in the control of hepatic zinc and zinc thionein levels in the rat. |
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| Keywords: | 1,8-ANS 1-anilinonaphthalene-8-sulfonate DTNB 5,5′-dithiobis (2-nitrobenzoate) 1,5-I-AEDANS N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid PEP phosphoenolpyruvate PEPCK phosphoenolpyruvate carboxykinase TPCK L-(1-tosylamido-2-phenyl)ethyl chloromethyl ketone |
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