Cloning, characterization and expression of the polypeptide release factor gene, eRF1, of Blepharisma japonicum |
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| Authors: | Wei Wang Bao-feng Chai Klaus Heckmann Ai-hua Liang |
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| Affiliation: | Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, P.R. China. |
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| Abstract: | The polypeptide release factor gene, eRF1, of Blepharisma japonicum (Bj-eRF1) was cloned and sequenced. Its coding region was 1314 base pairs and encodes a protein of 437 amino acids. The cloned gene was expressed in Escherichia coli and the recombinant Bj-eRF1 polypeptide was purified by Ni2+-nitrilotriacetic acid agarose and Superose12 chromatography. Pull-down analysis showed that the recombinant Bj-eRF1 interacts with the heterologously-expressed release factor, eRF3C, of Euplotes octocarinatus. |
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| Keywords: | Blepharisma japonicum ciliate expression interaction with eRF3 polypeptide release factor 1 |
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