Rapid Staining and Preservation of Cells Grown in Plastic Tissue Culture Flasks

Cells grown in plastic tissue culture flasks may easily be fixed and stained in large batches by first binding the flasks in groups of 6-8 and then applying all fluids with an automatic syringe, followed by decanting as required After staining, the cells are mounted in a mixture of USP mineral oil in isoamyl alcohol (2:1). In this way the cells are impregnated with a clearing and mounting agent which does not cause discoloration of the stains, does not dissolve the plastic, and is nondrying.