Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Trichoderma reesei FS10-C and Effect of Bioaugmentation on an Aged PAH-Contaminated Soil |
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| Authors: | Lunfang Yao Yongming Luo Peter Christie Wenting Ma Fang Liu |
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| Institution: | 1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China;2. College of Resources and Environmental Engineering, Guizhou University, Guiyang, China;3. Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China;4. College of Resources and Environmental Engineering, Guizhou University, Guiyang, China |
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| Abstract: | ABSTRACT?The co-metabolism of benzoa]pyrene (Ba]P) and the capacity of the fungus Trichoderma reesei FS10-C to bioremediate an aged polycyclic aromatic hydrocarbon (PAH)-contaminated soil were investigated. The fungal isolate removed about 54% of Ba]P (20 mg L?1) after 12 days of incubation with glucose (10 g L?1) supplementation as a co-metabolic substrate. Bioaugmented microcosms showed a 25% decrease in total PAH concentrations in soil after 28 days, and the degradation percentages of 3-, 4-, and 5(+6)-ring PAHs were 36%, 35%, and 25%, respectively. In addition, bioaugmented microcosms exhibited higher dehydrogenase (DHA) and fluorescein diacetate hydrolysis (FDAH) activities and increased average well-color development (AWCD), Shannon-Weaver index (H), and Simpson index (D) significantly. Principal component analysis (PCA) also distinguished clear differentiation between treatments, indicating that bioaugmentation restored the microbiological function of the PAH-contaminated soil. The results suggest that bioaugmentation by T. reesei FS10-C might be a promising bioremediation strategy for aged PAH-contaminated soils. |
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| Keywords: | bioaugmentation biodegradation PAHs soil contamination Trichoderma reesei FS10-C |
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