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Simple reversed-phase high-performance liquid chromatography quantitation of ganciclovir in human serum and urine
Authors:Theodore Page   Charles Sherwood  James D. Connor  Thomas Tarnowski
Affiliation:aDepartment of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA;bDepartment of Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA;cRoche Bioscience, Palo Alto, CA 94303, USA
Abstract:A fast, simple, and cost-effective HPLC method for the quantitation of the antiviral drug ganciclovir is described. The serum samples are extracted with perchloric acid and neutralized with potassium phosphate buffer, and urine samples are diluted with distilled water. A reversed-phase column with isocratic elution by 15 mM potassium phosphate buffer (pH 2.5) containing 0.25% acetonitrile is used to separate ganciclovir; quantitation is by UV absorbance at 254 nm. Total turnaround time is 22 min; more than 3000 samples can be run on a single column without loss of peak quality. The limit of quantitation is 0.05 μg/ml. Recoveries varied from 91 to 10% with coefficients of variation ranging from 0.387 to 7.95%.
Keywords:Ganciclovir
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