High-performance liquid chromatographic determination of midazolam in rat brain |
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| Authors: | Qibo Jiang Nancy Y. Walton Sonny Gunawan David M. Treiman |
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| Affiliation: | aNeurology and Research Services, Department of Veteran Affairs Medical Center, Los Angeles, CA 90073, USA;bDepartment of Neurology, UCLA School of Medicine, W127C, Building 304, Room E3-112, West Los Angeles DVA Medical Center, 11301 Wilshire Boulevard, Los Angeles, CA 90095-1769, USA |
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| Abstract: | A high-performance liquid chromatography method for the determination of midazolam in rat brain is described. Midazolam and the internal standard halazepam were extracted with toluene and analyzed isocratically on a reversed-phase column with a mobile phase consisting of methanol, acetonitrile and potassium phosphate buffer. Detection was monitored by ultraviolet absorption at 240 nm. The standard curves were linear over the range of 25--350 ng midazolam per 50 mg brain tissue. The day-to-day coefficient of variation ranged from 1.7 to 6.9%. The limit of quantification was 80 ng/g brain tissue. The method is rapid, simple and reproducible for brain analysis. |
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| Keywords: | Midazolam |
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