Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection |
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| Authors: | Arturo A. Vitale ,Carina C. Ferrari,Herná n Aldana,Jorge M. Affanni |
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| Affiliation: | aProplame-Conicet, Programa de Plantas Tóxicas y Medicinales, Metabolismo de Compuestos Sinteticos y Naturales, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina;bIneuci-Conicet, Instituto de Neurociencias, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
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| Abstract: | In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue. |
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| Keywords: | Melatonin |
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