Lactate dehydrogenase in abdominal muscle of crayfishOrconectes limosus and shrimpcrangon crangon (Decapoda: Crustacea): Properties and evolutionary relationship |
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| Authors: | Marek S Zie¸tara Jadwiga Gronczewska Krystyna Stachowiak Edward F Skorkowski |
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| Institution: | *Gdansk University Biological Station, 80-680 Gdansk-Sobieszewo, Poland;†Department of Organic Chemistry Gdansk University, Gdansk, Poland;‡Marine Biology Center Polish Academy of Sciences, Gdynia, Poland |
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| Abstract: | In crayfishOrconectes limosus and shrimpCrangon crangon abdominal muscle, lactate dehydrogenase (LDH, EC 1.1.1.27) is encoded by one locus. No polymorphism was detected. The enzymes were purified to homogeneity. The specific activities for purified crayfish and shrimp LDHs were 472 and 414 μmol NADH min−1 mg−1, respectively, at 30°C. Their physicochemical and kinetic properties did not resemble fish (Gadus morhua) LDH-A4 isoenzyme. Their amino acid composition indicated greater similarity to fish LDH-C4 isoenzymes. |
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| Keywords: | LDH crayfish shrimp decapods cod fish amino acid composition enzyme evolution |
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