Application of 2-halopyridinium salts as ultraviolet derivatization reagents and solid-phase extraction for determination of captopril in human plasma by high-performance liquid chromatography |
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| Authors: | Edward Bald ,Stanis?aw Sypniewski,Jó zef Drzewoski,Mariusz St?pień |
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| Affiliation: | 1. Department of Environmental Chemistry, University of Łódź, 163 Pomorska Str., 90-236 Łódź, Poland;2. Department of Clinical Pharmacology, Medical University of Łódź, 37139 Rewolucji 1905 Str., 90-214 Łódź, Poland;1. Université de Lorraine, UMR CNRS 7565, Structure et Réactivité des Systèmes Moléculaires Complexes, Equipe 5 (MIC), Campus Bridoux, rue du Général Delestraint, 57070 Metz Cedex, France;2. Université de Lorraine, UMR CNRS 7565, Structure et Réactivité des Systèmes Moléculaires Complexes, Equipe 3 (HECRIN), 1 Boulevard Arago, 57078 Metz Technopôle, France;3. Université de Lorraine, EA 4632-Laboratoire de Chimie et Physique Approche Multi-échelle des Milieux Complexes, 1 boulevard Arago, 57078 Metz Cedex 3, France;1. Department of Internal Medicine and Clinical Immunology, AP-HP, Hôpital Pitié-Salpétrière, DHU I2B, Immunopathology, Inflammation, Biotherapy, Paris, France;2. Université Pierre et Marie Curie, Paris VI, UMR CNRS 7211, INSERM U959, Hôpital Pitié-Salpétrière, Paris, France;3. Department of Biostatistics, Hôpital Saint-Louis, Paris, France;4. Department of Hepatology, Hôpital Necker, Paris, France;5. Department of Virology, Hôpital Pitié-Salpétrière, Paris, France;6. Department of Internal Medicine, Hôpital Lapeyronie, Montpellier, France;7. Department of Infectious Diseases, Hôpital Tenon, Paris, France;8. Department of Nephrology, Hôpital Européen George Pompidou, Paris, France;9. Department of Nephrology, Hôpital de la Robertsau, Strasbourg, France;10. Department of Internal Medicine, Centre Hospitalier de Nouvelle Calédonie, Nouméa, France;11. Department of Infectious Diseases, Hôpital Paul Brousse, Villejuif, France;12. Laboratory of Immunochemistry, Hôpital Pitié-Salpétrière, Paris, France;13. Department of Nephrology, Hôpital Tenon, Paris, France;14. Department of Internal Medicine, Hôpital Sud, Rennes, France;15. Department of Internal Medicine, Hôpital Croix Saint Simon, Paris, France;16. Department of Internal Medicine, Hôpital Bicêtre, Kremlin Bicêtre, France;1. Pediatric Surgery Department, Faculty of Medicine, Hospital São João, Porto, Portugal;2. Pediatric Surgery Department, Centro Hospitalar do Porto, Porto, Portugal;3. Pediatric Surgery Department, Hospital Pediátrico de Coimbra, Coimbra, Portugal;4. Pediatric Surgery Department, Centro Hospitalar Lisboa Central Hospital D. Estefânia, Lisboa, Portugal;1. Physics Program, Bard College, 30 Campus Rd, Annandale-on-Hudson, NY 12504, USA;2. Department of Physics, Florida Atlantic University, 777 Glades Rd, Boca Raton, FL 33431, USA;3. Institut für Quantengravitation, Universität Erlangen, Staudtstrasse 7, D-91058 Erlangen, Germany;4. Instytut Fizyki Teoretycznej, Uniwersytet Warszawski, ul. Pasteura 5, 02-093 Warszawa, Poland;5. Perimeter Institute for Theoretical Physics, 31 Caroline St N, Waterloo, Ontario N2L2Y5, Canada |
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| Abstract: | A high-performance liquid chromatographic method was developed for the determination of captopril in human plasma. 1-Benzyl-2-chloropyridinium bromide (BCPB) was used as a precolumn derivatizing reagent. The mercapto group of captopril was arylated by the reagent to generate a stable UV-sensitive product. The derivative was solid-phase extracted (SPE), separated on a C18 column using reversed-phase ion-paring chromatography and monitored by a spectrophotometric detector at 314 nm. The method enabled sensitive determination of captopril and its disulphides in human plasma in patients after oral administration. Disulphides of captopril with captopril itself and with endogenous thiol compounds are reduced with triphenylphosphine to form captopril, followed by derivatization with the same reagent. The quantification limit was 10 ng/ml. Calibration curves were prepared for human plasma samples spiked with captopril and captopril disulphide. The calibration curves were linear in the range of 10 to 500 ng/ml for captopril and 10 to 1000 ng/ml for captopril disulphide. |
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| Keywords: | Derivatization, LC solid-phase extraction Captopril 1-Benzyl-2-chloropyridinium bromide |
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